Page 75 - 中國動物保健雜誌第19卷第1期
P. 75

??研究

              doi:10.3969/j.issn.1008-4754.2017.1.038






                 小?瘟病毒卵?抗体被?免疫期研究



                                                 藏玉婷,王彬,宋?,丁?杰            *

                                  (哈?集?生物疫苗有限公司研?中心 黑?江哈?? 150069)

                摘要: 本??取 3 批??室?制的卵?抗体,??效价? 1?16,按 0.5 mL/ 只接种 1、3 日?易感??,
                分?在注射抗体后 1、3、5 和 7 日攻毒,?察攻毒保?情?。?果?示:1 日???注射抗体后 1 日攻毒
                保?率? 96.67%,3 日攻毒保?率? 100%,5 日攻毒保?率? 86.67%,7 日攻毒保?率? 76.67%;3 日?
                ??注射抗体后 1 日攻毒保?率? 100%,3 日攻毒保?率? 96.67%,5 日攻毒保?率? 86.67%,7 日攻
                毒保?率? 80%。以免疫攻毒保?率不低于 80%??准,本研究确定小?瘟病毒卵?抗体的被?免疫保
                ?期? 5 日, ????的延?,保?作用逐??弱。
                ???:小?瘟病毒;卵?抗体;??;被?免疫


                Research of Passive Immunity Periods of Goose Parvovirus Yolk Antibodies


                                       Zang Yuting,WangBin,SongYang,Ding Goujie      *
                            (Harbin Pharmaceutical Group Co Ltd Biological Vaccine,Harbin 150069,China)
              Abstract: 3 batches of the goose parvovirus yolk antibodies were produced in laboratory with the titer of AGAR
              diffusion test 1:16. The experiment groups were injected with 0.5ml goose parvovirus yolk antibodies for goslings

              that were 1 and 3 days of age. After 1、3、5 and 7 days, the experiment groups and the control groups were both at-
              tacked with goose parvovirus. The results showed that for 1 day goslings, the virus attack protection was 96.67% af-
              ter 1 day, it was 100% after 3 days, it was 86.67% after 5 days, it was 76.67% after 7 days; and for 3 days goslings, the
              virus attack protection was 100% after 1 day, it was 96.67% after 3 days, it was 86.67% after 5 days, it was 80% after
              7 days. In case of protective rate more than 80%, the research was determined that passive immunity period of goose
              parvovirus yolk antibodies was five days. As the extension of time, the protection of goslings was weakened.
              Keywords: Goose parvovirus; Yolk antibodies; Goslings; Passive immunity


                                                                         [3]
                  小?瘟是??一种?病急、症??重、死亡率                          ??意? 。本???用??室制?的 3 批小?瘟
              高的?染病,?病主要侵害出?后 4~20 日?的?                         病毒卵?抗体,????行抗体被?免疫作用持?
                                                                                                            [4]
              ?,也感染??,?播速度快,?病率和死亡率?高                           ???定,以确定小?瘟病毒卵?抗体被?免疫期 。
              ? 90%~100% 。早期?防?控制本病十分重要,常                       1 材料与方法
                           [1]
              以注射卵?抗体做被?免疫,?到?急?防的目的 。                          1.1 材料
                                                          [2]
              目前通?抗原免疫?蛋??得的抗小?瘟病毒特                             1.1.1 小?瘟病毒卵?抗体 哈?集?生物疫苗有限
              异性卵?抗体用于?防和治?小?瘟已取得了很                             公司研?中心制?,批?:201401、201402、201403,
              好的效果,使用卵?抗体做?急?防接种有?大的                            共? 3 批,瓶?量 100 mL,2~8 ℃保存,有效期?
                                                                18 ?月。??效价 ?1?16。
              作者?介:藏玉婷,?士,主要?事?用疫苗研制工作。
              * 通?作者: 丁?杰,?士,主要?事?用疫苗研制工作。                      1.1.2 小?瘟病毒 自行分离的小?瘟病毒 H 株, ?


                                                                                                                65
   70   71   72   73   74   75   76   77   78   79   80