Page 105 - 中國動物保健雜誌2017年第19卷第11期
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??研究
doi:10.3969/j.issn.1008-4754.2017.11.046
?多种病原混合感染的研究
???,李婷婷,?冰洋,王彩先,唐娟,丁??,詹?娥 *
(山西省??科?院畜牧??研究所 山西太原 030032)
摘 要: ?了确定山西太谷?某??蛋??病、死亡的原因且及治?方案的确定,通??床症??察、病理
剖?、病原的分离培?和 47 种生化??的生化?定,并??定出的病原菌?行?物敏感性??,其中使用
了?染性喉气管炎包涵体?查、?脂?散??、?物??以及新城疫的 RT-PCR ??等方法。 ?果表明:
分离菌?大?杆菌,??大霉素、安普霉素等?物敏感,并且有?染性喉气管炎病毒和新城疫病毒感染。?
??种情?,?合使用?物和疫苗控制住了病情,?群恢复健康。通?本案例警示?殖?在?物?病?程
中?全面考?多种病原混合感染的情?。
???: ?染性喉气管炎;新城疫;大?杆菌;?敏??; RT-PCR
Study on Mixed Infection of Multiple Pathogens of Chicken
Liu Huadong, Li Tingting, Lu Bingyang, Wang Caixian, Tang Juan, Ding Shurong, Zhan Lie
(Animal Husbandry and Veterinary Research Institute of Shanxi Academic of Agricultural Sciences,
Taiyuan, Shanxi, 030032)
Abstract: In order to ascertain the reasons of sickening and dying of laying hens in Shanxi Taigu county and then
find the treatments, this experiment used clinical observation, pathological examination, pathogen isolation and
biochemical identification of 47 kinds of biochemical reagents, meanwhile the drug sensitivity test was did to iden-
tify pathogenic bacteria, which included the examination of the inclusion body of infectious laryngotracheitis, agar
diffusion test, animal trial and RT-PCR test for Newcastle disease. The results showed that the isolated bacteria was
Escherichia coli, which was sensitive to gentamicin and Apramycin drug, and accompanied with infectious laryn-
gotracheitis virus and Newcastle disease virus infection. In this case, the combined utilization of drugs and vaccines
controlled the condition, and the flock recuperated the healthy. This alerted farms that a variety of mixed infection
should be considered once the animal is sick in livestock prodcution.
Keywords: infectious laryngotracheitis; Newcastle disease; Escherichia coli; drug sensitivity test; RT-PCR
2016 年 10 月 28 日山西省太谷?某??? 85 由于近期?生疾病,死亡?量?增,每天死亡?量
日?的蛋??生疾病。此???存?? 10,000 只, 30~40 只,且?量逐日增加。大群?精神??差,采
食量和?水量均下降。?主?? 5 只?病???者
研究室??,以下是?????容。
基金?目:山西省??科?院院重?攻??目(YGG1612);
1 ?床症?
山西省攻??目 (201603D221022-1)。
作者?介: ??? (1980-),男,助理研究?,?士,研究方向: 病?喜?,?翅下垂,伸??口呼吸,眼睛??,
畜禽?染病, E-mail:liuhd-2006@163.com 呈打盹?,反???, 拉??色水?稀便。
* 通?作者: 詹?娥 (1959-),女,研究?,本科,研究方向:畜 2 病理?化
禽?染病, E-mail:zhan7093767@126.com 剖?可?喉?水?且有?色干酪?分泌物堵
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