Page 107 - 中國動物保健雜誌2017年第19卷第6期
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??研究

              doi:10.3969/j.issn.1008-4754.2017.06.052





                    河南新??物犬、?与弓形虫病感染?系探?



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                            党耀宏 ,王?康 ,方?超 ,?萌 ,?永? ,苗? ,胡翠 ,?露文 ,?世?                            2*
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                 (1 新???院第一?床?院 河南新? 453000;2 新???院分子病原体??室 河南新? 453000)
                摘   要:目的:?了了解河南省新?市?物犬、?弓形虫感染情?,探???、性?与弓形虫感染?系,?弓形
                虫病的防治提供依据。方法: 2014 年 10 月 -2015 年 10 月直接采集?物??血,用酚??方法提取?物犬、
                ?血液中? DNA,?弓形虫 529 多拷?片段作?待?弓形虫序列,通??管一步法巢式 PCR 技??机?新
                ?市? 124 只?物犬、 54 只?物??行了弓形虫??。?果: 124 份?物犬?本中,弓形虫?性 13 份,?性率
                ? 10.48%(13/124),54 份??本中有弓形虫?性 28 份,?性率? 51.85%(28/54); ?血?本中, 雌性?性率
                63.16%(12/19),雄性?性率 47.83%(11/23);?各年?段中,小于 6 月??性率 66.67%(6/9),6 月 ~12 月??
                性率 75.00%(6/8),1 ? ~3 ??性率 60.00%(9/15),3 ?以上?性率 50.00%(1/2)。??:?物?弓形虫感染率

                高于?物犬;?物?性?之?弓形虫感染??著差异;??年?增?,?物?弓形虫感染率逐?下降。
                ???: 弓形虫;犬;?;巢式 PCR

                  Epidemiological Investigations of Toxoplasmosis in Pet Dogs and Cats in

                                          Xinxiang City of Henan Province

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                DANG Yaohong ,WANG Sukang ,FANG Pengchao ,ZHANG Meng ,ZHANG Yongle ,MIAO Shuo ,Hu
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                                               Cui ,ZHANG Luwen ,LIU Shiguo 2*
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                               (1.First Affiliated Hospital of Xinxiang Medical University,Xinxiang,453000;
                            2.Molecular pathogen laboratory of Xinxiang Medical University,Xinxiang,453000.)
                Abstract: Objectives :A epidemiological survey of Toxoplasma gondii infection in pet dogs and cats in Xinxiang
                City, Henan Province,to explore the relationship between animal age and gender with Toxoplasmosis, and to
                provide the basis for the prevention and treatment of Toxoplasmosis. Methods: Single tube nested PCR was used
                to detect the DNA fragment sequences of Toxoplasma gondii which is distilled from collecting venous blood of
                124 pet dogs and 54 pet cats by Phenol reagent in Xinxiang area during the period from October 2014 to October
                2015. Results: The results showed that the positive rate was 10.48%(13/124) in 124 pet dogs and 51.85%(28/54)
                in pet cats blood samples, in this pet cats blood samples, 63.16%(12/19) in the female and 47.83%(11/23)in
                male. All ages of cats, the positive rate was 66.67%(6/9) less than 6 months old, 75.00%(6/8)from 6 to 12 months
                old,60.00%(9/15) from 1 year old to 3 years old and 50.00%(1/2) above 3 years old. Conclusion: The Toxoplas-
                mosis positive rate of pet cats was significantly higher than pet dogs. There was no significant differences in Tox-
                oplasma gondii infection of pet cats among sexes. When the age of pet cat grows, the infection rate of Toxoplasma
                gondii is gradually declined.
                Key words: Toxoplasma gondii;dog;cat; Nested PCR.





              * 通?作者: ?世?, lsg1963@163.com


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