Page 107 - 中國動物保健雜誌2017年第19卷第6期

??研究

doi:10.3969/j.issn.1008-4754.2017.06.052

河南新??物犬、?与弓形虫病感染?系探?

1
1
党耀宏 ，王?康 ，方?超 ，?萌 ，?永? ，苗? ，胡翠 ，?露文 ，?世? 2*
1
1
2
1
1
1
（1 新???院第一?床?院 河南新? 453000；2 新???院分子病原体??室 河南新? 453000）
摘 要：目的：?了了解河南省新?市?物犬、?弓形虫感染情?，探???、性?与弓形虫感染?系，?弓形
虫病的防治提供依据。方法： 2014 年 10 月 -2015 年 10 月直接采集?物??血，用酚??方法提取?物犬、
?血液中? DNA，?弓形虫 529 多拷?片段作?待?弓形虫序列，通??管一步法巢式 PCR 技??机?新
?市? 124 只?物犬、 54 只?物??行了弓形虫??。?果： 124 份?物犬?本中，弓形虫?性 13 份，?性率
? 10.48%（13/124），54 份??本中有弓形虫?性 28 份，?性率? 51.85%（28/54）； ?血?本中， 雌性?性率
63.16%（12/19），雄性?性率 47.83%（11/23）；?各年?段中，小于 6 月??性率 66.67%（6/9），6 月 ~12 月??
性率 75.00%（6/8），1 ? ~3 ??性率 60.00%（9/15），3 ?以上?性率 50.00%(1/2)。??：?物?弓形虫感染率

高于?物犬；?物?性?之?弓形虫感染??著差异；??年?增?，?物?弓形虫感染率逐?下降。
???： 弓形虫；犬；?；巢式 PCR

Epidemiological Investigations of Toxoplasmosis in Pet Dogs and Cats in

Xinxiang City of Henan Province

1
1
1
1
1
1
DANG Yaohong ,WANG Sukang ,FANG Pengchao ,ZHANG Meng ,ZHANG Yongle ,MIAO Shuo ,Hu
1
Cui ,ZHANG Luwen ,LIU Shiguo 2*
2
(1.First Affiliated Hospital of Xinxiang Medical University,Xinxiang,453000;
2.Molecular pathogen laboratory of Xinxiang Medical University,Xinxiang,453000.)
Abstract: Objectives :A epidemiological survey of Toxoplasma gondii infection in pet dogs and cats in Xinxiang
City, Henan Province，to explore the relationship between animal age and gender with Toxoplasmosis, and to
provide the basis for the prevention and treatment of Toxoplasmosis. Methods: Single tube nested PCR was used
to detect the DNA fragment sequences of Toxoplasma gondii which is distilled from collecting venous blood of
124 pet dogs and 54 pet cats by Phenol reagent in Xinxiang area during the period from October 2014 to October
2015. Results: The results showed that the positive rate was 10.48%(13/124) in 124 pet dogs and 51.85%(28/54)
in pet cats blood samples, in this pet cats blood samples, 63.16%(12/19) in the female and 47.83%(11/23)in
male. All ages of cats, the positive rate was 66.67%(6/9) less than 6 months old, 75.00%(6/8)from 6 to 12 months
old，60.00%(9/15) from 1 year old to 3 years old and 50.00%(1/2) above 3 years old. Conclusion: The Toxoplas-
mosis positive rate of pet cats was significantly higher than pet dogs. There was no significant differences in Tox-
oplasma gondii infection of pet cats among sexes. When the age of pet cat grows, the infection rate of Toxoplasma
gondii is gradually declined.
Key words: Toxoplasma gondii;dog;cat; Nested PCR.

* 通?作者： ?世?， lsg1963@163.com

85